Products
VPS4B antibody
- Product Name
- VPS4B antibody
- Catalogue No.
- FNab09446
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- vacuolar protein sorting 4 homolog B
- Alternative Names
- MIG1 antibody, Protein SKD1 antibody, SKD1 antibody, VPS4 2 antibody, VPS42 antibody, VPS4B antibody
- UniProt ID
- O75351
- Observed MW
- 50 kDa
Application
- Tested Applications
- ELISA, WB, IF, IP
- Recommended dilution
- WB: 1:500-1:5000；IP: 1:500-1:5000；IF: 1:10-1:100
Validated Images
Y79 cells were subjected to SDS PAGE followed by western blot with FNab09446(VPS4B antibody) at dilution of 1:500
IP Result of anti-VPS4B (IP:FNab09446, 4ug; Detection:FNab09446 1:1000) with HeLa cells lysate 880ug.
- Background
- Involved in late steps of the endosomal multivesicular bodies(MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles(ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding(HIV-1 and other lentiviruses). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan(PubMed:22660413).
