Products
VPS18 antibody
- Product Name
- VPS18 antibody
- Catalogue No.
- FNab09429
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- vacuolar protein sorting 18 homolog
- Alternative Names
- HVPS18 antibody, KIAA1475 antibody, PEP3 antibody, VPS18 antibody
- UniProt ID
- Q9P253
- Observed MW
- 110 kDa
Application
- Tested Applications
- ELISA, IP, IHC, FC, IF
- Recommended dilution
- WB: 1:200-1:1000; IHC: 1:50-1:500; IP: 1:200-1:1000; IF: 1:20-1:200
Validated Images
HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab09429(VPS18 antibody) at dilution of 1:300
IP Result of anti-VPS18 (IP: FNab09429 4ug; Detection: FNab09429 1:300) with HeLa cells lysate 1000ug.
Immunohistochemical of paraffin-embedded human lung cancer using FNab09429(VPS18 antibody) at dilution of 1:100
Immunofluorescent analysis of A549 cells using FNab09429 (VPS18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations(PubMed:11382755, PubMed:23351085, PubMed:24554770, PubMed:25783203). Required for fusion of endosomes and autophagosomes with lysosomes(PubMed:25783203). Involved in dendrite development of Pukinje cells(By similarity).
