Products

anti- Vimentin antibody

FNab09410 Catalogue No.
Synonyms
FLJ36605, VIM, Vimentin
Host
Mouse
Reactivity
Human, Mouse, Rat
Isotype
IgG1
Tested Application
ELISA, WB, IHC
Product Information
Product Name
anti- Vimentin antibody
Catalogue No.
FNab09410
Size
100µg
Form
liquid
Purification
Protein A+G purification
Purity
≥95% as determined by SDS-PAGE
Host
Mouse
Clonality
monoclonal
Isotype
IgG1
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092) , suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
Immunogen Information
Immunogen
vimentin
Synonyms
FLJ36605, VIM, Vimentin
Observed MW
55 kDa
Application
Tested Application
ELISA, WB, IHC
Recommended dilution
WB :1:1000-1:10000; IHC: 1:20-1:2000; IF: 1:20-1:200
Uniprot information
UniProt ID
P08670
Images
Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using FNab09410 (Vimentin Antibody) at dilution of 1:500.
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab09410 (Vimentin Antibody) at dilution of 1:2000