Products

anti- Vimentin antibody

FNab09409 Catalogue No.
Synonyms
FLJ36605, VIM, Vimentin
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Tested Application
ELISA, WB, IHC, IF
Product Information
Product Name
anti- Vimentin antibody
Catalogue No.
FNab09409
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha(TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10(124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.
Immunogen Information
Immunogen
vimentin
Synonyms
FLJ36605, VIM, Vimentin
Observed MW
55 kDa
Application
Tested Application
ELISA, WB, IHC, IF
Recommended dilution
WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Uniprot information
UniProt ID
P08670
Images
Immunohistochemistry of paraffin-embedded human ovary cancer using FNab09409(VIM antibody) at dilution of 1:100
Immunofluorescent analysis of HepG2 cells, using FNab09409(VIM antibody) at 1:25 dilution 
Hela cells were subjected to SDS PAGE followed by western blot with FNab09409(VIM antibody) at dilution of 1:1000