Products
UPF3B antibody
- Product Name
- UPF3B antibody
- Catalogue No.
- FNab09272
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- UPF3 regulator of nonsense transcripts homolog B(yeast)
- Alternative Names
- HUPF3B antibody, hUpf3p X antibody, MRXS14 antibody, RENT3B antibody, UPF3B antibody, UPF3X antibody
- UniProt ID
- Q9BZI7
- Observed MW
- 58 kDa
Application
- Tested Applications
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:500-1:5000; IHC：1:20-1:200
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab09272(UPF3B antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human testis slide using FNab09272(UPF3B Antibody) at dilution of 1:50
- Background
- Involved in nonsense-mediated decay(NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex(EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex(including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF2 stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA upstream of exon-exon junctions. In vitro, stimulates translation; the function is independent of association with UPF2 and components of the EJC core.
