Products
UBE2O antibody
- Product Name
- UBE2O antibody
- Catalogue No.
- FNab09181
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- ubiquitin-conjugating enzyme E2O
- Alternative Names
- E2-230K antibody, FLJ12878 antibody, KIAA1734 antibody, UBE2O antibody, Ubiquitin carrier protein O antibody, Ubiquitin protein ligase O antibody
- UniProt ID
- Q9C0C9
- Observed MW
- 141 kDa
Application
- Tested Applications
- ELISA, WB
- Recommended dilution
- WB: 1:200-1:2000
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab09181(UBE2O antibody) at dilution of 1:500
- Background
- E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins(PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity(PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis(PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal(NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location(PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASH1, leading to promote endosomal F-actin assembly(PubMed:23452853).
