Products
U2AF35 antibody
- Product Name
- U2AF35 antibody
- Catalogue No.
- FNab09138
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 1E9
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- U2 small nuclear RNA auxiliary factor 1
- Alternative Names
- DKFZp313J1712 antibody, FP793 antibody, RN antibody, RNU2AF1 antibody, U2AF1 antibody, U2AF35 antibody, U2AFBP antibody
- UniProt ID
- Q01081
- Observed MW
- 38 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:5000; IHC: 1:20-1:200; IP: 1:200-1:2000
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab09138(U2AF35 Antibody) at dilution of 1:1000
IP Result of anti-U2AF35 (IP:FNab09138, 3ug; Detection:FNab09138 1:500) with HeLa cells lysate 3000ug.
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab09138(U2AF35 Antibody) at dilution of 1:50
- Background
- U2 auxiliary factor(U2AF), comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2(RNU2) small nuclear RNA auxiliary factor 1(U2AF1,synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1)is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder.
