Products
TRIM27 antibody
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Research Area:
Synonyms: | Ret finger protein antibody, RFP antibody, RING finger protein 76 antibody, RNF76 antibody, TRIM27 antibody, tripartite motif containing 27 antibody, Zinc finger protein RFP antibody | ||
Catalogue No.: | FNab08977 | Reactivity: | Human, Mouse, Rat |
Host: | Rabbit | Tested Application: | ELISA, WB, IHC, IP, IF |
Clonality: | polyclonal | Isotype: | IgG |
- SPECIFICATIONS
- Product Name
- TRIM27 antibody
- Catalogue No.
- FNab08977
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- tripartite motif-containing 27
- Alternative Names
- Ret finger protein antibody, RFP antibody, RING finger protein 76 antibody, RNF76 antibody, TRIM27 antibody, tripartite motif containing 27 antibody, Zinc finger protein RFP antibody
- UniProt ID
- P14373
- Observed MW
- 58 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP, IF
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:5000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab08977(TRIM27 antibody) at dilution of 1:1000
IP Result of anti-TRIM27 (IP:FNab08977, 4ug; Detection:FNab08977 1:1000) with mouse testis tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human lung cancer using FNab08977(TRIM27 antibody) at dilution of 1:100
Immunofluorescent analysis of HeLa cells using FNab08977 (TRIM27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASH1, leading to promote endosomal F-actin assembly(PubMed:23452853). Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.