Products

TRIM25 antibody

Synonyms:EFP antibody, RING finger protein 147 antibody, RNF147 antibody, TRIM25 antibody, tripartite motif containing 25 antibody, Z147 antibody, Zinc finger protein 147 antibody, ZNF147 antibody
Catalogue No.:FNab08976Reativity:Human, Mouse
Host:RabbitTested Application:ELISA, WB, IF, IP
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
TRIM25 antibody
Catalogue No.
FNab08976
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
tripartite motif-containing 25
Alternative Names
EFP antibody, RING finger protein 147 antibody, RNF147 antibody, TRIM25 antibody, tripartite motif containing 25 antibody, Z147 antibody, Zinc finger protein 147 antibody, ZNF147 antibody
UniProt ID
Q14258
Observed MW
71 kDa
Application
Tested Applications
ELISA, WB, IF, IP
Recommended dilution
WB: 1:200-1:2000; IP: 1:200-1:2000; IF: 1:20-1:200
Validated Images
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab08976(TRIM25 antibody) at dilution of 1:500
IP Result of anti-TRIM25 (IP:FNab08976, 4ug; Detection:FNab08976 1:600) with K-562 cells lysate 3200ug.
Background
Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma(SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3(PubMed:22452784).