Products
TPX2 antibody
- Product Name
- TPX2 antibody
- Catalogue No.
- FNab08910
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- TPX2, microtubule-associated, homolog(Xenopus laevis)
- Alternative Names
- C20orf1 antibody, C20orf2 antibody, DIL2 antibody, HCA519 antibody
- UniProt ID
- Q9ULW0
- Observed MW
- 100 kDa
Application
- Tested Applications
- ELISA, WB, IF, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IF: 1:20-1:200
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab08910(TPX2 antibody) at dilution of 1:5000
IP Result of anti-TPX2 (IP:FNab08910, 4ug; Detection:FNab08910 1:800) with HeLa cells lysate 880ug.
- Background
- Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules(PubMed:18663142, PubMed:19208764). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation(PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin-alpha(PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation(PubMed:26165940).
