Products
TP53 antibody
- Product Name
- TP53 antibody
- Catalogue No.
- FNab08878
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- tumor protein p53
- Alternative Names
- Cellular tumor antigen p53|Antigen NY-CO-13|Phosphoprotein p53|Tumor suppressor p53|TP53|P53 antibody
- UniProt ID
- P04637
- Observed MW
- 53 kDa
Application
- Tested Applications
- ELISA, IHC, IP, WB, IF
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:50-1:500; IF: 1:20-1:200
Validated Images
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab08878(P53 antibody) at dilution of 1:1000
IP Result of anti-TP53 (IP: FNab08878, 2ug; Detection: FNab08878 1:1000) with A431 cells lysate 1000ug.
Immunohistochemistry of paraffin-embedded human ovary tumor tissue using FNab08878(P53 antibody) at dilution of 1:100
- Background
- Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21(lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2(PubMed:24051492).
