Products

TP53 antibody

Synonyms:Cellular tumor antigen p53 antibody, FLJ92943 antibody, LFS1 antibody, P53 antibody, Phosphoprotein p53 antibody, TP53 antibody, TRP53 antibody, tumor protein p53 antibody, Tumor suppressor p53 antibody
Catalogue No.:FNab08878Reativity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, IHC, IP, WB, IF
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
TP53 antibody
Catalogue No.
FNab08878
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
tumor protein p53
Alternative Names
Cellular tumor antigen p53 antibody, FLJ92943 antibody, LFS1 antibody, P53 antibody, Phosphoprotein p53 antibody, TP53 antibody, TRP53 antibody, tumor protein p53 antibody, Tumor suppressor p53 antibody
UniProt ID
P04637
Observed MW
53 kDa
Application
Tested Applications
ELISA, IHC, IP, WB, IF
Recommended dilution
WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:50-1:500; IF: 1:20-1:200
Validated Images
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab08878(P53 antibody) at dilution of 1:1000
IP Result of anti-TP53 (IP: FNab08878, 2ug; Detection: FNab08878 1:1000) with A431 cells lysate 1000ug.
Immunohistochemistry of paraffin-embedded human ovary tumor tissue using FNab08878(P53 antibody) at dilution of 1:100
Background
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21(lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2(PubMed:24051492).