Products
TOP1MT antibody
- Product Name
- TOP1MT antibody
- Catalogue No.
- FNab08866
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- topoisomerase(DNA) I, mitochondrial
- UniProt ID
- Q969P6
- Observed MW
- 70 kDa
Application
- Tested Applications
- ELISA, WB
- Recommended dilution
- WB: 1:500-1:2000
Validated Images
COLO 320 cells were subjected to SDS PAGE followed by western blot with FNab08866(TOP1MT Antibody) at dilution of 1:1000
- Background
- Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone(By similarity).
