PC-3 cells were subjected to SDS PAGE followed by western blot with FNab08854(TOLLIP antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human gliomas using FNab08854(TOLLIP antibody) at dilution of 1:100
Immunofluorescent analysis of PC-3 cells using FNab08854 (TOLLIP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
Background
Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity(PubMed:11751856). Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates(PubMed:25042851).