Various lysates were subjected to SDS PAGE followed by western blot with FNab08627(TFEB antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human placenta tissue using FNab08627(TFEB antibody) at dilution of 1:100
Immunofluorescence analysis of HeLa cells using TFEB antibody (FNab08627) at dilution of 1:100.
Background
TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calcualted molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa.