Products
TDP1 antibody
- Product Name
- TDP1 antibody
- Catalogue No.
- FNab08572
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- tyrosyl-DNA phosphodiesterase 1
- UniProt ID
- Q9NUW8
- Observed MW
- 68 kDa
Application
- Tested Applications
- ELISA, WB, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:2000
Validated Images
MCF7 cells were subjected to SDS PAGE followed by western blot with FNab08572(TDP1 antibody) at dilution of 1:1000
IP Result of anti-TDP1 (IP:FNab08572, 4ug; Detection:FNab08572 1:1000) with HeLa cells lysate 1200ug.
- Background
- DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate.
