HeLa cells were subjected to SDS PAGE followed by western blot with FNab08546(TCEA3 antibody) at dilution of 1:500
Background
Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.