Products
Synaptotagmin-1 antibody
- Product Name
- Synaptotagmin-1 antibody
- Catalogue No.
- FNab08425
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- synaptotagmin I
- Alternative Names
- DKFZp781D2042 antibody, P65 antibody, SVP65 antibody, Synaptotagmin 1 antibody, synaptotagmin I antibody, SYT antibody, SYT1 antibody, SytI antibody
- UniProt ID
- P21579
- Observed MW
- 65 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:50-1:500
Validated Images
human liver tissue were subjected to SDS PAGE followed by western blot with FNab08425(SYT1 antibody) at dilution of 1:1000
IP Result of anti-Synaptotagmin-1 (IP:FNab08425, 4ug; Detection:FNab08425 1:500) with mouse testis tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human liver cancer using FNab08425(SYT1 antibody) at dilution of 1:100
Immunofluorescent analysis of ( -20℃ Ethanol) fixed HepG2 cells using FNab08425 (Synaptotagmin-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis(PMID: 8058779). SYT1(synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue(PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse(PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth(PMID: 15492212; 11078930; 10336114). This antibody recognizes endogenous SYT1, which has an experimentally determined molecular mass of 65-68 kDa. An additional band of 47 kDa could also be detected, which is corresponding to the calculated molecular weight of SYT1.
