A549 cells were subjected to SDS PAGE followed by western blot with FNab08229(SRP14 antibody) at dilution of 1:800
IP Result of anti-SRP14 (IP: FNab08229, 4ug; Detection: FNab08229 1:300) with A549 cells lysate 1600ug.
Background
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.