Products
SPOP antibody
- Product Name
- SPOP antibody
- Catalogue No.
- FNab08189
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- speckle-type POZ protein
- Alternative Names
- HIB homolog 1 antibody, Roadkill homolog 1 antibody, speckle type POZ protein antibody, SPOP antibody, TEF2 antibody
- UniProt ID
- O43791
- Observed MW
- 42 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:50-1:500; IP : 1:500-1:1000
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab08189 (SPOP antibody) at dilution of 1:500
IP Result of anti-SPOP (IP:FNab08189, 3ug; Detection:FNab08189 1:500) with HeLa cell lysate 3000ug.
Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using FNab08189( SPOP Antibody) at dilution of 1:200
- Background
- Component of a cullin-RING-based BCR(BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of H2AFY and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR(BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL.
