SPHK2 antibody

Catalogue No.:FNab08175Reactivity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC, IP
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
SPHK2 antibody
Catalogue No.
FNab08175
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
sphingosine kinase 2
UniProt ID
Q9NRA0
Observed MW
65-70 kDa
Application
Tested Applications
ELISA, WB, IHC, IP
Recommended dilution
WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with FNab08175(SPHK2 antibody) at dilution of 1:300
IP Result of anti-SPHK2 (IP:17096-1-AP, 4ug; Detection:17096-1-AP 1:800) with mouse liver tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using FNab08175(SPHK2 Antibody) at dilution of 1:200
Background
Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate(SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.