Products
SP1 antibody
- Product Name
- SP1 antibody
- Catalogue No.
- FNab08131
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- Sp1 transcription factor
- Alternative Names
- SP1 antibody, Sp1 transcription factor antibody, Transcription factor Sp1 antibody, TSFP1 antibody
- UniProt ID
- P08047
- Observed MW
- 90kd
Application
- Tested Applications
- ELISA, WB, IP, IHC
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:50-1:500; IF: 1:20-1:200
Validated Images
Jurkat cells were subjected to SDS PAGE followed by western blot with FNab08131(SP1 antibody) at dilution of 1:500
IP Result of anti-SP1 (IP: FNab08131, 4ug; Detection: FNab08131 1:500) with A431 cells lysate 2500ug.
Immunohistochemistry of paraffin-embedded human lung cancer tissue using FNab08131(SP1 antibody) at dilution of 1:50
- Background
- Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications(phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component ARNTL/BMAL1.
