Products
SIRT5 antibody
- Product Name
- SIRT5 antibody
- Catalogue No.
- FNab07883
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- sirtuin(silent mating type information regulation 2 homolog) 5
- Alternative Names
- FLJ36950 antibody, SIR2 like protein 5 antibody, SIR2L5 antibody, SIRT5 antibody
- UniProt ID
- Q9NXA8
- Observed MW
- 33 kDa
Application
- Tested Applications
- ELISA, WB, IP
- Recommended dilution
- WB: 1:500-1:1000; IP: 1:500-1:1000; IF: 1:50-1:500
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab07883(SIRT5 antibody) at dilution of 1:600
IP Result of anti-SIRT5 (IP: FNab07883, 4ug; Detection: FNab07883 1:500) with mouse liver tissue lysate 6000ug.
- Background
- NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins(PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting(PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species(PubMed:24140062). Modulates ketogenesis through the desuccinylation and activation of HMGCS2(By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c(CYCS) and a number of other proteins in vitro such as UOX.
