Products
SHARPIN antibody
- Product Name
- SHARPIN antibody
- Catalogue No.
- FNab07838
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- SHANK-associated RH domain interactor
- Alternative Names
- SIPL1 antibody
- UniProt ID
- Q9H0F6
- Observed MW
- 40 kDa
Application
- Tested Applications
- ELISA, WB, IP, IHC
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
Raji cells were subjected to SDS PAGE followed by western blot with FNab07838(SHARPIN antibody) at dilution of 1:500
IP Result of anti-SHARPIN (IP:FNab07838, 3ug; Detection:FNab07838 1:1500) with Raji cells lysate 2520ug.
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using FNab07838(SHARPIN Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
- Background
- Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex(TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.
