HeLa cells were subjected to SDS PAGE followed by western blot with FNab07780(SF3B4 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human pancreas cancer using FNab07780(SF3B4 antibody) at dilution of 1:50
Background
Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence(BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.