Products
SATB1 antibody
- Product Name
- SATB1 antibody
- Catalogue No.
- FNab07615
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- SATB homeobox 1
- UniProt ID
- Q01826
- Observed MW
- 100 kDa
Application
- Tested Applications
- ELISA, WB, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:2000
Validated Images
human brain tissue were subjected to SDS PAGE followed by western blot with FNab07615(SATB1 antibody) at dilution of 1:4000
IP Result of anti-SATB1 (IP:FNab07615, 4ug; Detection:FNab07615 1:500) with HEK-293 cells lysate 2000ug.
- Background
- Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma(By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence(CSBS), at nuclear matrix-or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions(MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes(e.g. PML at the MHC-I locus) and also by recruiting corepressors(HDACs) or coactivators(HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta-and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region(UR) of cytomegalovirus(CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA(interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly up-regulating metastasis-associated genes while down-regulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.
