Products
RRP8 antibody
- Product Name
- RRP8 antibody
- Catalogue No.
- FNab07501
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- ribosomal RNA processing 8, methyltransferase, homolog(yeast)
- Alternative Names
- KIAA0409 antibody, NML antibody
- UniProt ID
- O43159
- Observed MW
- 51 kDa
Application
- Tested Applications
- ELISA, WB, IF, IHC
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
human brain tissue were subjected to SDS PAGE followed by western blot with FNab07501(RRP8 antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human skin using FNab07501(RRP8 antibody) at dilution of 1:100
- Background
- Essential component of the eNoSC(energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9'(H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.
