Products
ROCK2 antibody
- Product Name
- ROCK2 antibody
- Catalogue No.
- FNab07378
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- Rho-associated, coiled-coil containing protein kinase 2
- Alternative Names
- KIAA0619 antibody, p164 ROCK 2 antibody, Rho kinase 2 antibody, ROCK2 antibody
- UniProt ID
- O75116
- Observed MW
- 161 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:1000-1:10000; IP: 1:200-1:2000;; IHC: 1:20-1:200; IF: 1:10-1:100
Validated Images
HT-1080 cells were subjected to SDS PAGE followed by western blot with FNab07378(ROCK2 antibody) at dilution of 1:1000
IP Result of anti-ROCK2(middle) (IP:FNab07378, 5ug; Detection:FNab07378 1:700) with HeLa cells lysate 1320ug.
Immunohistochemistry of paraffin-embedded human liver using FNab07378(ROCK2 antibody) at dilution of 1:100
- Background
- Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.
