Products

RLIM antibody

Synonyms:RNF12 antibody
Catalogue No.:FNab07320Reativity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IP
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
RLIM antibody
Catalogue No.
FNab07320
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
ring finger protein, LIM domain interacting
Alternative Names
RNF12 antibody
UniProt ID
Q9NVW2
Observed MW
75 kDa
Application
Tested Applications
ELISA, WB, IP
Recommended dilution
WB: 1:500-1:2000; IP: 1:200-1:2000
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab07320(RLIM antibody) at dilution of 1:500
IP Result of anti-RLIM (IP:FNab07320, 4ug; Detection:FNab07320 1:500) with HeLa cells lysate 1520ug.
Background
E3 ubiquitin-protein ligase. Acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex-related dosage differences of X-encoded genes in somatic cells of female placental mammals.