Products
REST antibody
- Product Name
- REST antibody
- Catalogue No.
- FNab07244
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- RE1-silencing transcription factor
- Alternative Names
- NRSF antibody, REST antibody, X2 box repressor antibody, XBR antibody
- UniProt ID
- Q13127
- Observed MW
- 122 kDa, 200kd
Application
- Tested Applications
- ELISA, WB, IP
- Recommended dilution
- WB: 1:500-1:1000; IP: 1:500-1:2000
Validated Images
HeLa cells were subjected to SDS PAGE followed by western blot with FNab07244(REST Antibody) at dilution of 1:1000
IP Result of anti-REST (IP: FNab07244, 3ug; Detection: FNab07244 1:2000) with HeLa cells lysate 2500ug.
- Background
- Transcriptional repressor which binds neuron-restrictive silencer element(NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression.
