HeLa cells were subjected to SDS PAGE followed by western blot with FNab07079(RAD51 Antibody) at dilution of 1:1000
IP Result of anti-RAD51 (IP:FNab07079, 3ug; Detection:FNab07079 1:1000) with mouse testis tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using FNab07079(RAD51 Antibody) at dilution of 1:200
Background
Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange(PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.