Products
Rac1 antibody
- Product Name
- Rac1 antibody
- Catalogue No.
- FNab07065
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 5D9
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- ras-related C3 botulinum toxin substrate 1(rho family, small GTP binding protein Rac1)
- Alternative Names
- MIG5 antibody, p21 Rac1 antibody, RAC1 antibody, RAC1b antibody, Ras like protein TC25 antibody, TC 25 antibody, TC25 antibody
- UniProt ID
- P63000
- Observed MW
- 21KD
Application
- Tested Applications
- ELISA, WB,IHC, IP,IF
- Recommended dilution
- WB: 1:1000-1:4000; IP: 1:200-1:2000; IHC: 1:50-1:500; IF: 1:50-1:500
Validated Images
HSCT6 cells were subjected to SDS PAGE followed by western blot with FNab07065(Rac1 antibody) at dilution of 1:2000
IP Result of anti-RAC1 (IP:FNab07065, 3ug; Detection:FNab07065 1:700) with HL-60 cells lysate 4000ug.
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using FNab07065(RAC1 Antibody) at dilution of 1:400
Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using FNab07065 (Rac1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)
- Background
- RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
