Products

anti- PUM2 antibody

FNab06955 Catalogue No.
Synonyms
KIAA0235, PUMH2
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Tested Application
ELISA, WB
Product Information
Product Name
anti- PUM2 antibody
Catalogue No.
FNab06955
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element(PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element(NRE)(, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation(PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs(PubMed:18776931, PubMed:22345517). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation(PubMed:22345517). Plays a role in cytoplasmic sensing of viral infection(PubMed:25340845). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD(non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm(PubMed:26724866). May regulate DCUN1D3 mRNA levels(PubMed:25349211). May support proliferation and self-renewal of stem cells.
Immunogen Information
Immunogen
pumilio homolog 2(Drosophila)
Synonyms
KIAA0235, PUMH2
Observed MW
140 kDa
Application
Tested Application
ELISA, WB
Recommended dilution
WB: 1:500-1:2000
Uniprot information
UniProt ID
Q8TB72
Images
mouse testis tissue were subjected to SDS PAGE followed by western blot with FNab06955( PUM2 Antibody) at dilution of 1:600