HeLa cells were subjected to SDS PAGE followed by western blot with FNab06799(PHB antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human liver cancer using FNab06799(PHB antibody) at dilution of 1:50
Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using FNab06799(PHB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
Background
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.