Products
PRKACB antibody
- Product Name
- PRKACB antibody
- Catalogue No.
- FNab06778
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- protein kinase, cAMP-dependent, catalytic, beta
- UniProt ID
- P22694
- Observed MW
- 46-55 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
HT-1080 cells were subjected to SDS PAGE followed by western blot with FNab06778(PRKACB antibody) at dilution of 1:1000
IP Result of anti-PRKACB (IP:FNab06778, 4ug; Detection:FNab06778 1:500) with mouse brain tissue lysate 2640ug.
Immunohistochemistry of paraffin-embedded human medulloblastoma using FNab06778(PRKACB antibody) at dilution of 1:50
- Background
- Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis.
