Products

PPP4R2 antibody

Catalogue No.:FNab06734Reativity:Human
Host:RabbitTested Application:ELISA, WB, IHC
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
PPP4R2 antibody
Catalogue No.
FNab06734
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
protein phosphatase 4, regulatory subunit 2
UniProt ID
Q9NY27
Observed MW
65 kDa
Application
Tested Applications
ELISA, WB, IHC
Recommended dilution
WB: 1:500-1:2000; IHC: 1:20-1:200
Validated Images
A549 cells were subjected to SDS PAGE followed by western blot with FNab06734(PPP4R2 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab06734(PPP4R2 Antibody) at dilution of 1:50
Background
Regulatory subunit of serine/threonine-protein phosphatase 4(PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140'(gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair.