Products
POLI antibody
- Product Name
- POLI antibody
- Catalogue No.
- FNab06612
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- polymerase(DNA directed) iota
- Alternative Names
- DNA polymerase iota antibody, Eta2 antibody, POLI antibody, polymerase (DNA directed) iota antibody, RAD30 homolog B antibody, RAD30B antibody, RAD3OB antibody
- UniProt ID
- Q9UNA4
- Observed MW
- 87 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:20-1:200
Validated Images
mouse testis tissue were subjected to SDS PAGE followed by western blot with FNab06612(POLI antibody) at dilution of 1:800
IP Result of anti-POLI (IP:FNab06612, 4ug; Detection:FNab06612 1:1000) with mouse testis tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human pancreas cancer using FNab06612(POLI antibody) at dilution of 1:100
- Background
- Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
