Products
POFUT1 antibody
- Product Name
- POFUT1 antibody
- Catalogue No.
- FNab06602
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- protein O-fucosyltransferase 1
- Alternative Names
- FUT12 antibody, KIAA0180 antibody
- UniProt ID
- Q9H488
- Observed MW
- 44 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab06602(POFUT1 antibody) at dilution of 1:500
IP Result of anti-POFUT1 (IP:FNab06602, 4ug; Detection:FNab06602 1:500) with HepG2 cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human liver cancer using FNab06602(POFUT1 antibody) at dilution of 1:50
- Background
- Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors(AChRs).
