PLOD3 antibody

Catalogue No.:FNab06553Reactivity:Human, Mouse, Rat
Host:RabbitTested Application:ELISA, WB, IHC, IP, IF
Clonality:polyclonalIsotype:IgG
  • SPECIFICATIONS
Product Name
PLOD3 antibody
Catalogue No.
FNab06553
Size
100μg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
Immunogen
procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
UniProt ID
O60568
Observed MW
80-85 kDa
Application
Tested Applications
ELISA, WB, IHC, IP, IF
Recommended dilution
WB: 1:500-1:2000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
Validated Images
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab06553(PLOD3 antibody) at dilution of 1:800
IP Result of anti-PLOD3 (IP:FNab06553, 3ug; Detection:FNab06553 1:500) with HepG2 cells lysate 6000ug.
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using FNab06553(PLOD3 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Background
Forms hydroxylysine residues in-Xaa-Lys-Gly-sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.