Products
PHD3 antibody
- Product Name
- PHD3 antibody
- Catalogue No.
- FNab06377
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- egl nine homolog 3
- Alternative Names
- Egl nine homolog 3 antibody, EGLN3 antibody, FLJ21620 antibody, HIF PH3 antibody, HIF prolyl hydroxylase 3 antibody, HIFPH3 antibody, HPH 1 antibody, HPH 3 antibody, PHD3 antibody
- UniProt ID
- Q9H6Z9
- Observed MW
- 28kd
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:20-1:200
Validated Images
HT-1080 cells were subjected to SDS PAGE followed by western blot with FNab06377(EGLN3 antibody) at dilution of 1:1000
IP Result of anti-PHD3 (IP:FNab06377, 4ug; Detection:FNab06377 1:500) with A375 cells lysate 3600ug.
Immunohistochemistry of paraffin-embedded human brain using FNab06377(EGLN3 antibody) at dilution of 1:50
- Background
- EGLN3, also named as HPH-1, HIF-PH3, HPH-3 and PHD3, is a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor(HIF) alpha proteins. It hydroxylates a specific proline found in each of the oxygen-dependent degradation(ODD) domains(N-terminal, NODD, and C-terminal, CODD) of HIF1A. It is a regulator of cardiomyocyte and neuronal apoptosis. EGLN3 can be a prognostic marker for gastric cancer.
