Products
PD-L1/CD274 antibody
- Product Name
- PD-L1/CD274 antibody
- Catalogue No.
- FNab06280
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- CD274 molecule
- Alternative Names
- B7 H antibody, B7 H1 antibody, B7 homolog 1 antibody, B7H1 antibody, CD274 antibody, CD274 molecule antibody, PD L1 antibody, PDCD1 ligand 1 antibody, PDCD1L1 antibody, PDCD1LG1 antibody, PDL1 antibody, PD-L1 antibody, PD-L1/CD274 antibody, Programmed death ligand 1 antibody
- UniProt ID
- Q9NZQ7
- Observed MW
- 50 kDa, 70 kDa
Application
- Tested Applications
- ELISA, WB, IF, IHC, IP, FC
- Recommended dilution
- WB: 1:200-1:1000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:10-1:100
Validated Images
mouse heart tissue were subjected to SDS PAGE followed by western blot with FNab06280(PD-L1/CD274 Antibody) at dilution of 1:300
IP Result of anti-PD-L1/CD274 (IP:FNab06280, 4ug; Detection:FNab06280 1:1000) with mouse heart tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded mouse heart tissue slide using FNab06280(PD-L1/CD274 Antibody) at dilution of 1:50
Immunofluorescent analysis of HEK-293 cells using FNab06280 (PD-L1/CD274 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- Programmed cell death ligand 1(CD274, or B7-H1, PD-L1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. CD274 is suggested a negative regulator of T and B cell, and play important role in mediating tolerance of lymphocytes to self-antigens. It also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33 kDa, the apparent molecular weight has been reported as 45-70 kDa, suggesting probable glycosylation(PMID: 18760278; 16493058).?
