Products
PDK1 antibody
- Product Name
- PDK1 antibody
- Catalogue No.
- FNab06275
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- pyruvate dehydrogenase kinase, isozyme 1
- Alternative Names
- PDH kinase 1 antibody, PDHK1 antibody, PDK1 antibody, Pyruvate dehydrogenase kinase isoform 1 antibody
- UniProt ID
- Q15118
- Observed MW
- 50kd
Application
- Tested Applications
- ELISA, IHC, WB, IP
- Recommended dilution
- WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:50-1:500
Validated Images
mouse liver tissue were subjected to SDS PAGE followed by western blot with FNab06275(PDK1 Antibody) at dilution of 1:1000
IP Result of anti-PDK1 (IP:FNab06275, 6ug; Detection:FNab06275 1:1000) with NIH/3T3 cells lysate 1200ug.
Immunohistochemistry of paraffin-embedded human liver using FNab06275(PDK1 antibody) at dilution of 1:100
- Background
- Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
