HeLa cells were subjected to SDS PAGE followed by western blot with FNab06210(PCK1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using FNab06210(PCK1 antibody) at dilution of 1:200
Background
This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized.