Products

anti- PALB2 antibody

FNab06125 Catalogue No.
Synonyms
DKFZp667I166, DKFZp686E1054, FANCN, FLJ21816, PALB2, partner and localizer of BRCA2
Host
Rabbit
Reactivity
Human
Isotype
IgG
Tested Application
ELISA, WB, IHC
Product Information
Product Name
anti- PALB2 antibody
Catalogue No.
FNab06125
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Plays a critical role in homologous recombination repair(HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A(RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Via its WD repeats is proposed to scaffold a HR complex containing RAD51C and BRCA2 which is thought to play a role in HR-mediated DNA repair. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with BRCA2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity.
Immunogen Information
Immunogen
partner and localizer of BRCA2
Synonyms
DKFZp667I166, DKFZp686E1054, FANCN, FLJ21816, PALB2, partner and localizer of BRCA2
Observed MW
131kd
Application
Tested Application
ELISA, WB, IHC
Recommended dilution
WB: 1:500-1:2000; IHC: 1:20-1:200
Uniprot information
UniProt ID
Q86YC2
Images
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab06125(PALB2 Antibody) at dilution of 1:100
various lysates were subjected to SDS PAGE followed by western blot with FNab06125(PALB2 antibody) at dilution of 1:500