anti- PALB2 antibody
FNab06125
Catalogue No.
- Synonyms:
- DKFZp667I166, DKFZp686E1054, FANCN, FLJ21816, PALB2, partner and localizer of BRCA2
- Host:
- Rabbit
- Reactivity:
- Human
- Isotype:
- IgG
- Tested Application:
- ELISA, WB, IHC
Product Information
- Product Name
- anti- PALB2 antibody
- Catalogue No.
- FNab06125
- Size
- 100µg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Host
- Rabbit
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
- Plays a critical role in homologous recombination repair(HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A(RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Via its WD repeats is proposed to scaffold a HR complex containing RAD51C and BRCA2 which is thought to play a role in HR-mediated DNA repair. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with BRCA2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity.
Immunogen Information
- Immunogen
- partner and localizer of BRCA2
- Synonyms
- DKFZp667I166, DKFZp686E1054, FANCN, FLJ21816, PALB2, partner and localizer of BRCA2
- Observed MW
- 131kd
Application
- Tested Application
- ELISA, WB, IHC
- Recommended dilution
- WB: 1:500-1:2000; IHC: 1:20-1:200
Uniprot information
- UniProt ID
- Q86YC2
Images
- Image 1
-
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab06125(PALB2 Antibody) at dilution of 1:100
- Image 4
-
various lysates were subjected to SDS PAGE followed by western blot with FNab06125(PALB2 antibody) at dilution of 1:500