Products

anti- PAF1 antibody

FNab06106 Catalogue No.
Synonyms
PD2
Host
Rabbit
Reactivity
Human
Isotype
IgG
Tested Application
ELISA, WB, IHC, IP
Product Information
Product Name
anti- PAF1 antibody
Catalogue No.
FNab06106
Size
100µg
Form
liquid
Purification
Immunogen affinity purified
Purity
≥95% as determined by SDS-PAGE
Host
Rabbit
Clonality
polyclonal
Isotype
IgG
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Background
Component of the PAF1 complex(PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'-and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4'(H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B(H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors. Has oncogenic activity in vivo and in vitro.
Immunogen Information
Immunogen
Paf1, RNA polymerase II associated factor, homolog(S. cerevisiae)
Synonyms
PD2
Observed MW
80 kDa
Application
Tested Application
ELISA, WB, IHC, IP
Recommended dilution
WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Uniprot information
UniProt ID
Q8N7H5
Images
Immunohistochemistry of paraffin-embedded human ovary tumor using FNab06106(PAF1 antibody) at dilution of 1:100
IP Result of anti-PAF1 (IP:FNab06106, 3ug; Detection:FNab06106 1:1000) with HeLa cells lysate 3000ug.
HepG2 cells were subjected to SDS PAGE followed by western blot with FNab06106(PAF1 antibody) at dilution of 1:1000