HeLa cells were subjected to SDS PAGE followed by western blot with FNab05887(NUB1 antibody) at dilution of 1:600
IP Result of anti-NUB1 (IP: FNab05887, 3ug; Detection: FNab05887 1:600) with HEK-293 cells lysate 2500ug.
Background
Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.