HepG2 cells cells were subjected to SDS PAGE followed by western blot with FNab05870(NSUN2 antibody) at dilution of 1:5000
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using FNab05870(NSUN2 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunofluorescent analysis of Hela cells using FNab05870 (NSUN2 antibody) at dilution of 1:25
Background
RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine(m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.