Products
NRG1 antibody
- Product Name
- NRG1 antibody
- Catalogue No.
- FNab05856
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- neuregulin 1
- Alternative Names
- GGF antibody, HGL antibody, HRGA antibody, NDF antibody, SMDF antibody
- UniProt ID
- Q02297
- Observed MW
- 55 kDa and 72 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IF, IP
- Recommended dilution
- WB: 1:500-1:1000; IHC: 1:50-1:200 IP:1:500-1:2000; IF: 1:50-1:500
Validated Images
human liver tissue were subjected to SDS PAGE followed by western blot with FNab05856(NRG1 antibody) at dilution of 1:250
IP Result of anti-NRG1 (IP:FNab05856, 4ug; Detection:FNab05856 1:1000) with mouse liver tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human breast cancer using FNab05856(NRG1 antibody) at dilution of 1:100
Immunofluorescent analysis of HepG2 cells using FNab05856 (NRG1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
- Background
- Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4(PubMed:10867024, PubMed:7902537). Binds to ERBB3(PubMed:20682778). Acts as a ligand for integrins and binds(via EGF domain) to integrins ITGAV:ITGB3 or ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1(PubMed:20682778). Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons(By similarity).
