Products
NR5A1 antibody
- Product Name
- NR5A1 antibody
- Catalogue No.
- FNab05847
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- nuclear receptor subfamily 5, group A, member 1
- Alternative Names
- AD4BP antibody, FTZF1 antibody, SF1 antibody
- UniProt ID
- Q13285
- Observed MW
- 53 kDa
Application
- Tested Applications
- ELISA, WB, IHC, IP
- Recommended dilution
- WB: 1:500-1:5000; IP: 1:200-1:1000; IHC: 1:20-1:200
Validated Images
mouse ovary tissue were subjected to SDS PAGE followed by western blot with FNab05847(NR5A1 antibody) at dilution of 1:1000
IP Result of anti-NR5A1 (IP: FNab05847, 4ug; Detection: FNab05847 1:300) with A2780 cells lysate 960ug.
Immunohistochemistry of paraffin-embedded human liver using FNab05847(NR5A1 antibody) at dilution of 1:100
- Background
- Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine(By similarity). Binds phospholipids with a phosphatidylinositol(PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
