HeLa cells were subjected to SDS PAGE followed by western blot with FNab05806(NOX4 antibody) at dilution of 1:600
IP Result of anti-NOX4 (IP:FNab05806, 3ug; Detection:FNab05806 1:1000) with HEK-293 cells lysate 1700ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using FNab05806(NOX4 Antibody) at dilution of 1:200
Immunofluorescent analysis of BxPC-3 cells using FNab05806 (NOX4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)
Background
Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation.