Products
NGEF antibody
- Product Name
- NGEF antibody
- Catalogue No.
- FNab05720
- Size
- 100μg
- Form
- liquid
- Purification
- Immunogen affinity purified
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- polyclonal
- Isotype
- IgG
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- neuronal guanine nucleotide exchange factor
- UniProt ID
- Q8N5V2
- Observed MW
- 75 kDa
Application
- Tested Applications
- ELISA, WB, IP, IHC
- Recommended dilution
- WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:50-1:500
Validated Images
mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab05720(NGEF antibody) at dilution of 1:500
IP Result of anti-NGEF (IP:FNab05720, 4ug; Detection:FNab05720 1:500) with mouse brain tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human brain tissue slide using FNab05720( NGEF antibody) at dilution of 1:200
- Background
- Acts as a guanine nucleotide exchange factor(GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension(By similarity).
